α-L-Fucosidase (AFU) Acitivity Assay Kit (E-BC-K762-M)
For research use only.
| Detection Principle | α-L-fucosidase (AFU) is an acid hydrolase that exists in organisms and widely exists in tissues, cells, blood and body fluids. AFU is involved in the metabolism of glycoproteins, glycolipids and oligosaccharides, and plays a key role in cell differentiation, apoptosis, inflammation and host-pathogen interaction. AFU catalyzes the hydrolysis of the colorless substrate nitrophenyl-α-L-fucoside to produce yellow nitrophenol with a significant absorption peak at 405 nm, and its absorbance is positively correlated with the content of nitrophenol. The activity of AFU can be calculated by measuring the change of absorbance value at 405 nm. |
| Synonyms | AFU |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (400-420 nm, optimum wavelength: 405 nm) |
| Research Area | Liver And Renal Function |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.5 U/L |
| Detection Range | 0.5-80 U/L |
| Precision | inter-assay CV: 8.4% | intra-assay CV: 3.7% |
| Sample Volume | 20 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat plasma | 1 |
| 10% Rat liver tissue homogenate | 1-3 |
| 10% Mouse liver tissue homogenate | 1-3 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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