Sialic Acid (SA) Colorimetric Assay Kit (E-BC-K068-M)
For research use only.
| Detection Principle | Sialic acid forms a purplish red complex with methyl resorcinol in the presence of oxidant. And the absorbance conforms to Lambert-Beer's law. The content of sialic acid can be calculated by measuring the OD value at 560 nm. |
| Synonyms | SA |
| Sample Type | Serum,Plasma,Saliva,Urine,Hydrothorax,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (510-570 nm, optimum wavelength: 560 nm) |
| Research Area | Other |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 0.03 mmol/L |
| Detection Range | 0.03-7 mmol/L |
| Precision | inter-assay CV: 6.1% | intra-assay CV: 0.04 |
| Sample Volume | 25 μL |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat serum | 1 |
| Mouse serum | 1 |
| Porcine serum | 1 |
| Human plasma | 1 |
| Rat plasma | 1 |
| Mouse plasma | 1 |
| Human saliva | 1 |
| Human urine | 1 |
| Human hydrothorax | 1 |
| 10% Plant tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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