Hydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit (Fenton Method) (E-BC-K042-M)

Name: Hydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit (Fenton Method)
Brand: Elabscience
SKU: E-BC-K042-M
Price: 220.0 USD
Availability: InStock

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Hydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit (Fenton Method) - 1

All	Size	Price	Qty
	96T	$ 320.00	- +
	48T	$ 220.00	- +

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Detection Principle	he Fenton reaction is the most common chemical reaction that generates hydroxyl radicals (OH·). The amount of hydrogen peroxide is directly proportional to the amount of OH· produced by the Fenton reaction. After the electron accepter is given, adding griess reagent to produce the red substance, which is directly proportional to the amount of OH·. The substances contained in the sample that can eliminate hydroxyl free radical can consume OH·. The remaining OH· is detected to reflect hydroxyl free radical scavenging capacity.
Sample Type	Serum,Plasma,Animal and plant tissue,Liquid
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (540-560 nm, optimum wavelength: 550 nm)
Research Area	Oxidative Stress
Other Reagents Required	Normal saline (0.9% NaCl)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	1.46 U/mL
Detection Range	1.46-82.89 U/mL
Precision	inter-assay CV: 4.49-7.19% \| intra-assay CV: 2.19-5.42%
Sample Volume	100 μL
Assay Time	1 h 10 min

Cat.No.	Product Name	Sizes
E-BC-K807-M	DPPH Free Radical Scavenging Capacity Colorimetric Assay Kit	96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	8-32
Human plasma	32-64
Mouse serum	16-32
10% Rat lung tissue homogenate	32-64

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.