α-Hydroxybutyrate Dehydrogenase (α-HBDH) Activity Colorimetric Assay Kit (E-BC-K1102-M)
For research use only.
| Detection Principle |
α-Hydroxybutyrate Dehydrogenase (α-HBDH) is a general term for Lactic Dehydrogenase (LDH) isoenzymes LDH 1 and LDH 2, and is one of the five components of the myocardial enzyme spectrum. The increase of serum creatinine is common in myocardial infarction, liver parenchymal cell lesions, rheumatic myocarditis, viral myocarditis, hemolytic anemia and other diseases. α-HBDH catalyzes the reduction of α-ketobutyric acid to α-hydroxybutyric acid in the presence of reduced coenzyme Ⅰ (NADH), and NADH is oxidized to NAD+ at a rate proportional to the enzyme activity. The activity of α-HBDH can be calculated by monitoring the rate of NADH reduction at 340 nm. |
| Sample Type | Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (340 nm) |
| Research Area | Metabolic Diseases , Liver And Renal Function |
| Other Reagents Required | PBS (0.01 M，pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 5.97 U/L |
| Detection Range | 5.97-1313.73 U/L |
| Precision | inter-assay CV: 2.3-3.6% | intra-assay CV: 2.4-7.4% |
| Sample Volume | 10 μL |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Mouse plasma | 1 |
| Human plasma | 1 |
| Rat plasma | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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