Hydrogen sulfide (H2S) Colorimetric Assay Kit (Direct Method) (E-BC-K894-M)
For research use only.
| Detection Principle | H2S can be absorbed by acetate solution to form ZnS, which can form methylene blue in the presence of Fe3+ and chromogenic agent. Methylene blue has a maximum absorption peak at 680 nm. H2S content can be calculated indirectly by measuring the OD value at 680 nm. |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (670-690 nm, optimum wavelength: 680 nm) |
| Research Area | Metabolic Diseases |
| Other Reagents Required | Double distilled water, Normal Saline (0.9% NaCl) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.97 μmol/L |
| Detection Range | 1.97-100 μmol/L |
| Precision | inter-assay CV: 4.5-7.7% | intra-assay CV: 2.6-4.4% |
| Sample Volume | 50 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human plasma | 1 |
| Mouse plasma | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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