Nitric Oxide (NO) Colorimetric Assay Kit (Nitrate Reductase Method) (E-BC-K135-M)
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For research use only.
| Detection Principle | Nitric oxide (NO) has active chemical properties and is quickly metabolized into NO2- and NO3- in the body, while NO2- is further transformed into NO3-. This method uses nitrate reductase specifically to reduce NO3- to NO2-, and the concentration of NO is measured by the color depth. |
| Synonyms | NO |
| Sample Type | Serum,Plasma,Urine,Tissue,Cell,Supernatant of Cell sample |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (520-550 nm, optimum wavelength: 530 nm) |
| Research Area | Oxidative Stress |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.38 μmol/L |
| Detection Range | 1.38-40 μmol/L |
| Precision | inter-assay CV: 0.08 | intra-assay CV: 0.06 |
| Sample Volume | 80-200 μL |
| Assay Time | 1 h 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human urine | 20-30 |
| Mouse serum | 1-3 |
| Chicken plasma | 1 |
| Rat urine | 20-30 |
| 10% Rat brain tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Vegetable leaf tissue homogenate | 1 |
| 1 × 10^6 Jurkat cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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