Free Fatty Acids (NEFA/FFA) Fluorometric Assay Kit (E-BC-F039)
For research use only.
| Detection Principle | Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids. |
| Synonyms | FFA;NEFA |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/590 nm) |
| Research Area | Metabolic Diseases , Glycolysis And Lipid Metabolism , Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.20 μmol/L |
| Detection Range | 1.20-100 μmol/L |
| Precision | inter-assay CV: 5.1% | intra-assay CV: 4.1% |
| Sample Volume | 50 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 10-20 |
| Rat serum | 200-400 |
| Mouse plasma | 100-300 |
| Rabbit serum | 100-300 |
| 10% Rat liver tissue homogenate | 200-400 |
| 10% Mouse kidney tissue homogenate | 200-400 |
| 10% Rat brain tissue homogenate | 200-400 |
| 10% Rat lung tissue homogenate | 200-400 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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