Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit (E-BC-K013-S)
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For research use only.
| Detection Principle | Under the condition of weak acidity, non-esterified free fatty acids (NEFA) react with nantokite to form copper soap, which has a specific absorption peak at 715 nm. The content of NEFA can be calculated by measuring the OD value at 715 nm. |
| Synonyms | NEFA |
| Sample Type | Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (715 nm) |
| Research Area | Glycolysis And Lipid Metabolism, Metabolic Diseases, Energy Metabolism |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.05 mmol/L |
| Detection Range | 0.05-2.0 mmol/L |
| Precision | inter-assay CV: 6.1% | intra-assay CV: 2.3% |
| Sample Volume | 0.2 mL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Rat liver tissue homogenate | 1-3 |
| Rat heart tissue homogenate | 1 |
| Rat kidney tissue homogenate | 1 |
| Mouse liver tissue homogenate | 1-3 |
| Rat serum | 1 |
| Mouse serum | 1 |
| Human serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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