Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit (Enzyme Method) (E-BC-K792-M)
For research use only.
| Detection Principle | Free fatty acids (NEFA/FFA) generate acyl-CoA under the action of acyl synthase, then acyl-CoA generates hydrogen peroxide under the oxidation of acyl oxidase. Hydrogen peroxide reacts in the presence of enzymes and chromogens, producing a red substance with maximum absorption at 546 nm. The amount of this red product is proportional to the content of free fatty acids. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (540-550 nm, optimum wavelength: 546 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Metabolic Diseases , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), Double distilled water |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 0.14 mmol/L |
| Detection Range | 0.14-4 mmol/L |
| Precision | inter-assay CV: 2.5-6.1% | intra-assay CV: 1.5-4.5% |
| Sample Volume | 10 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1-2 |
| Mouse serum | 4-6 |
| Rat serum | 4-6 |
| Bovine serum | 1-2 |
| 10% Rat liver tissue homogenate | 1-4 |
| 10% Rat heart tissue homogenate | 1-4 |
| 10% Mouse liver tissue homogenate | 1 |
| 3 × 10^6 293T cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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