Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit (E-BC-F151)
For research use only.
| Detection Principle |
Dihydroxyacetone phosphate (DHAP) is an intermediate product present in the glycolysis and gluconeogenesis processes of living organisms. Most animals, due to the absence of the glyoxylate cycle, cannot convert fatty acids into glucose like plants. They can only convert the glycerol in fats into glucose through DHAP or enter the glycolysis pathway. Detection principle: The products formed after a series of enzymatic reactions of DHAP can cause the fluorescent probe to emit fluorescence. The fluorescence value can be detected using a fluorescence microplate reader at the maximum excitation wavelength of 535 nm and the maximum emission wavelength of 587 nm to calculate the content of DHAP. |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Energy Metabolism |
| Other Reagents Required | PBS (0.01 M, pH 7.4), 1 mol/L Perchloric acid, 3 mol/L KHCO3 |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.63 μmol/L |
| Detection Range | 0.63-100 μmol/L |
| Precision | inter-assay CV: 1.9-4.7% | intra-assay CV: 1.1-3.3% |
| Sample Volume | 60 μL |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse spleen tissue homogenate | 1 |
| Mouse serum | 1 |
| Mouse plasma | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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