Cystathionine-β-synthase (CBS) Activity Colorimetric Assay Kit (E-BC-K811-M)
For research use only.
| Detection Principle | Cystathionine-β-synthase (CBS) can participate in the production of hydrogen sulfide, a gas signaling molecule, and play an important role in cell metabolism. The principle of this kit is that CBS and enzyme catalyzed substrates can convert NADH into NAD through enzyme-linked reaction. By detecting the change of NADH per unit time, the amount of CBS enzyme activity in the sample can be calculated. |
| Sample Type | Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (330-350 nm, optimum wavelength: 340 nm) |
| Research Area | Oxidative Stress , Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.005 U/L |
| Detection Range | 0.005-20.0 U/L |
| Precision | inter-assay CV: 5.2-10.1% | intra-assay CV: 2.5-4.9% |
| Sample Volume | 20 μL(cell/tissue homogenate) |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse kidney tissue homogenization | 1 |
| 10% Mouse brain tissue homogenization | 1 |
| 10% Mouse heart tissue homogenization | 1 |
| 10% Mouse liver tissue homogenization | 1 |
| 10% Mouse lung tissue homogenization | 1-5 |
| 1×10^6 293T cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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