Cell Hydrogen Sulfide (H2S) Fluorometric Assay Kit (E-BC-F103)
For research use only.
| Detection Principle |
Hydrogen Sulfide (H2S), as a key signal transduction gas molecule in cells and organisms, is involved in numerous physiological and pathological processes such as vascular relaxation and nerve transmission. The detection of intracellular H2S can deeply explore its specific mechanism and role in cellular signal transduction. Given that abnormal intracellular H2S concentration is associated with various diseases such as diabetes and liver cirrhosis, the detection of intracellular H2S can play an auxiliary role in the early diagnosis of diseases, condition monitoring, and evaluation of treatment effects. The detection principle of this kit: the probe produce green fluorescence with H2S after entering the cell. The level of H2S in cells can be detected by measuring the fluorescence intensity. |
| Sample Type | Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader, Fluorescence microscope, Flow cytometry |
| Research Area | Oxidative Stress |
| Other Reagents Required | PBS (0.01 M, pH 7.4) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Precision | inter-assay CV: 1.0-9.5% | intra-assay CV: 0.9-3.0% |
| Sample Volume | 0.1 mL |
| Assay Time | 1 h 30 min |
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