Caspase 8 Activity Detection Substrate for Flow Cytometry (E-CK-A488)
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For research use only.
| Detection Principle | The Caspase 8 Activity Detection Substrate for Flow Cytometry are a novel fluorogenic dye that couple the caspase 8 recognition sequence (IETD) to a high affinity DNA dye, which have cell membrane permeability and can penetrate the plasma membrane into the cytoplasm. The substrate itself has no fluorescence and has a charge exclusion effect with DNA. During cell apoptosis, caspase-8 cleaves the substrate and releases high affinity DNA dyes, which bind to DNA to produce strong fluorescence, thereby detecting caspase-8 activity and visualizing the morphological changes of the cell nucleus during apoptosis. |
| Detection Method | Fluorometric method, Flow cytometry |
| Sample Type | Cell samples |
| Assay Time | 30 min |
| Detection Instrument | Flow Cytometer;Fluorescence microscope |
| Ex/Em | Caspase 8: Ex/Em=490nm/535nm |
| Channel Set | FITC |
| Other Reagents Required | PBS, cell culture medium |
| Storage | This product can be stored at -20℃ for 1 year in the dark. |
| Expiration date | 12 months |
| Shipping | Ice bag |
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