Caspase 3/7 Activity Detection Substrate for Flow Cytometry (E-CK-A483)
For research use only.
| Detection Principle | The Caspase 3/7 Substrates（Green）are based on novel fluorogenic DNA dyes that have been coupled to the caspase 3/7 recognition sequence (DEVD),which is both non-fluorescent and nonfunctional as a DNA dye.When it rapidly crosses cell membranes to enter the cytoplasm and cleaved by caspase 3/7 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the Caspase 3/7 substrates allow detection of caspase 3/7 activity and visualization of apoptotic nuclear morphology simultaneously. |
| Detection Method | Fluorometric method, Flow cytometry |
| Sample Type | Cell samples |
| Assay Time | 0.5 hour |
| Detection Instrument | Flow Cytometer |
| Ex/Em | Caspase 3: Ex/Em=490nm/535nm |
| Channel Set | FITC |
| Other Reagents Required | PBS, cell culture medium, fetal bovine serum |
| Storage | This product can be stored at 2~8℃ for 1 year in the dark. |
| Expiration date | 12 months |
| Shipping | Ice bag |
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