Ca2+-ATPase Activity Assay Kit (E-BC-K212-S)

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All	Size	Price	Qty
	100Assays	$ 260.00
	50Assays	$ 180.00

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Detection Principle	ATPase exists on the membrane of tissue cells and organelles. It is a kind of protease on the biological membrane which plays an important role in material transport, energy conversion and information transmission. Ca²⁺ which participates in the regulation of different enzyme systems and cell activities plays many important roles in cells. The flow of Ca²⁺ depends on the Ca²⁺ -ATPase on the cell membrane, and Ca²⁺ -ATPase consumes ATP to generate the energy needed for ion transport.
Synonyms	Ca2+-ATPase
Sample Type	Animal tissue
Detection Method	Colorimetric method
Detection Instrument	Spectrophotometer (660 nm)
Research Area	Oxidative Stress
Other Reagents Required	Normal saline (0.9% NaCl)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.8 U/g wet weight
Detection Range	0.8-41 U/g wet weight
Precision	inter-assay CV: 8.8% \| intra-assay CV: 4.1%
Sample Volume	400 μL(tissue/cell homogenate)
Assay Time	50 min

Cat.No.	Product Name	Sizes
E-BC-K122-S	H+K+-ATPase Activity Assay Kit	200Assays , 100Assays
E-BC-K539-M	Na+k+-ATPase Activity Assay Kit	96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Rat liver tissue homogenate	5-8
10% Rat heart tissue homogenate	5-8
10% Rat kidney tissue homogenate	5-8
10% Mouse liver tissue homogenate	1
10% Rat lung tissue homogenate	5-8
10% Rat brain tissue homogenate	2-3

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.