H+K+-ATPase Activity Assay Kit (E-BC-K122-S)

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	200Assays	$ 300.00
	100Assays	$ 180.00

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Detection Principle	ATPase can decompose ATP to produce ADP and inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. The inorganic phosphorus reacts with ammonium molybdate in acidic solution to form ammonium molybdate compound, which is reduced with reducing agent to form molybdenum blue, and has absorption peak at 660 nm. Determine the concentration of molybdenum blue to calculate the amount of inorganic phosphorus.
Sample Type	Animal tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Spectrophotometer (660 nm)
Research Area	Oxidative Stress
Other Reagents Required	Normal saline (0.9% NaCl)
Storage	This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Valid Period	12 months
Precision	inter-assay CV: 9.8% \| intra-assay CV: 4.4%
Sample Volume	200 μL(tissue/cell homogenate)
Assay Time	1 h

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Animal tissue homogenate	5
GES-1 cells (2.52mg/mL)	2

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.