Alanine (Ala) Colorimetric Assay Kit (E-BC-K839-M)
For research use only.
| Detection Principle |
Alanine (Ala) is a non-essential amino acid, which is one of the 20 basic amino acids that make up proteins. Ala has a high content in human blood and plays an important role in human metabolic activities such as glucose-alanine cycle. It can also help the nervous system conduction and plays an important role in maintaining human health. The detection principle of this kit is as follows: alanine is decomposed by enzymes to generate NADH, which is catalyzed by electron carrier to form a chromogenic substance with WST-8. The chromogenic substance has a maximum absorption at the wavelength of 450 nm, and the Ala content in the sample is calculated by measuring the OD value at 450 nm and the standard curve. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 10.65 μmol/L |
| Detection Range | 10.65-500 μmol/L |
| Precision | inter-assay CV: 4.8-8.4% | intra-assay CV: 2.2-2.8% |
| Sample Volume | 40 μL |
| Assay Time | 1 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 3-10 |
| Mouse serum | 2-10 |
| Guinea pig plasma | 2-10 |
| Pig serum | 2-10 |
| 10% Mouse liver tissue homogenate | 3-10 |
| 10% Mouse kindey tissue homogenate | 2-10 |
| 1×10^6 Hela cells | 1 |
| 1×10^6 RAW264.7 cells | 1 |
| 1×10^6 Jurkat cells | 1 |
| 1×10^6 293T cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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