Acyl-CoA Synthetase (ACS) Activity Colorimetric Assay Kit (E-BC-K779-M)
For research use only.
| Detection Principle |
Acyl-CoA synthase (ACS) is an important enzyme that is widely present in living organisms and participates in the metabolic process of fatty acids. It is an enzyme necessary for lipid synthesis, fatty acid catabolism and membrane remodeling, and can also function as transcriptional activator, allosteric inhibitor or precursor of inflammatory mediators. In the medical field, mutations or abnormal expression of ACS may be associated with various diseases, such as diabetes and obesity. The detection principle of this kit: The ACS catalyzes the substrate, and the generated product reacts with the chromogenic agent to produce a purple substance, which has the largest absorption peak at 555 nm. Therefore, the absorbance at 555 nm can be measured to calculate the amount of product generated, thereby calculating the activity of ACS. |
| Sample Type | Serum,Plasma,Animal and plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (550-560 nm, optimum wavelength: 555 nm) |
| Research Area | Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.004 U/L |
| Detection Range | 0.004-0.107 U/L |
| Precision | inter-assay CV: 3.8-8.9% | intra-assay CV: 2.0-3.6% |
| Sample Volume | 20 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human plasma | 1 |
| Mouse serum | 1 |
| 10% Mouse liver tissue homogenate | 1-4 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Mouse leg muscle tissue homogenate | 1 |
| 10% Mouse spleen tissue homogenate | 1 |
| 10% Corn seed tissue homogenate | 1 |
| 1×10^6 Molt-4 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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