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Effective Solutions for Panel Design Challenges in Flow Cytometry

Source: Elabscience®Published: Nov 14,2024

Welcome to the final lecture in our Flow Cytometry Panel Design series - Effective Solutions for Panel Design Challenges in Flow Cytometry?

Today, we're tackling one of the biggest challenges: insufficient instrument channels. Ever found yourself needing to analyze Th1/Th2/Th17 cells but only having four channels? We've got the solution!

In this video, we'll explore:

● The importance of understanding marker roles in multicolor experiments.

● How to use panel splitting to effectively manage limited channels.

● Leveraging “lin” (lineage) markers to exclude unwanted cells.

● Prioritizing dye characteristics over traditional panel design rules.

Dive into our video on Panel Design and tackle those frustrating challenges like a pro!