Flow Cytometry
Effective Solutions for Panel Design Challenges in Flow Cytometry
Source: Elabscience®Published: Nov 14,2024
Welcome to the final lecture in our Flow Cytometry Panel Design series - Effective Solutions for Panel Design Challenges in Flow Cytometry?
Today, we're tackling one of the biggest challenges: insufficient instrument channels. Ever found yourself needing to analyze Th1/Th2/Th17 cells but only having four channels? We've got the solution!
In this video, we'll explore:
● The importance of understanding marker roles in multicolor experiments.
● How to use panel splitting to effectively manage limited channels.
● Leveraging “lin” (lineage) markers to exclude unwanted cells.
● Prioritizing dye characteristics over traditional panel design rules.
Dive into our video on Panel Design and tackle those frustrating challenges like a pro!