Flow Cytometry Panel Design Basic Knowledge of Fluorescence Part 2

Welcome back to Elabscience's Flow Cytometry Classroom!

Ever wondered why panel design is crucial in flow cytometry? Curious about fluorescence spectra and how to adjust fluorescence compensation?

In this second part of our series, we will learn:

● Fluorescence Compensation: Learn how to adjust for spectral overlap between fluorochromes like FITC and PE to ensure accurate results.

● Detection Principles: Understand how flow cytometers detect fluorescence using filters like 530/30 for FITC and 585/42 for PE.

● Optimizing Panel Design: Discover the importance of matching fluorochrome brightness to protein expression levels using the Staining Index (SI).

● Fluorochrome Properties: Get tips on selecting the right fluorochromes for your experiments, especially for intracellular or nuclear factor detection.

From handling spectral overlaps to choosing stable fluorochromes for different conditions, this session equips you with essential knowledge to enhance your flow cytometry experiments.