Preparation of Separating Gel

Source: Elabscience^®Published: Dec 04,2018

Components	Sample volume of each component corresponding to different separation gel volumes
Components	5 mL	10 mL	15 mL	20 mL	25 mL	30 mL	40 mL	50 mL
6% Gel
H₂O	2.6	5.3	7.9	10.6	13.2	15.9	21.2	26.5
30% Acrylamide	1	2	3	4	5	6	8	10
1.5M Tris-HCl(pH8.8)	1.3	2.5	3.8	5	6.3	7.5	10	12.5
10% SDS	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
10% (NH₄)₂S₂O₈	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
TEMED	0.004	0.008	0.012	0.016	0.02	0.024	0.032	0.04
8% Gel
H₂O	2.3	4.6	6.9	9.3	11.5	13.9	18.5	23.2
30% Acrylamide	1.3	2.5	4	5.3	6.7	8	10.7	13.3
1.5M Tris-HCl(pH8.8)	1.3	2.5	3.8	5	6.3	7.5	10	12.5
10% SDS	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
10% (NH₄)₂S₂O₈	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
TEMED	0.003	0.006	0.009	0.012	0.015	0.018	0.024	0.03
10% Gel
H₂O	1.9	4	5.9	7.9	9.9	11.9	15.9	19.8
30% Acrylamide	1.7	3.3	5	6.7	8.3	10	13.3	16.7
1.5M Tris-HCl(pH8.8)	1.3	2.5	3.8	5	6.3	7.5	10	12.5
10% SDS	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
10% (NH₄)₂S₂O₈	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
TEMED	0.002	0.004	0.006	0.008	0.01	0.012	0.016	0.02
12% Gel
H₂O	1.6	3.3	4.9	6.6	8.2	9.9	15.9	16.5
30% Acrylamide	2	4	6	8	10	12	13.3	20
1.5M Tris-HCl(pH8.8)	1.3	2.5	3.8	5	6.3	7.5	10	12.5
10% SDS	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
10% (NH₄)₂S₂O₈	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
TEMED	0.002	0.004	0.006	0.008	0.01	0.012	0.016	0.02
15% Gel
H₂O	1.1	2.3	3.4	4.6	5.7	6.9	9.2	11.5
30% Acrylamide	2.5	5	7.5	10	12.5	15	20	25
1.5M Tris-HCl(pH8.8)	1.3	2.5	3.8	5	6.3	7.5	10	12.5
10% SDS	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
10% (NH₄)₂S₂O₈	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5