ZBTB7A Polyclonal Antibody (E-AB-53231)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2, NIH/3T3 |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human ZBTB7A |
| Abbre | ZBTB7A |
| Synonyms | DKFZp547O146, FBI-1, FBI1, Factor binding IST protein 1, Factor that binds to inducer of short transcripts protein 1, HIV-1 1st-binding protein 1, HIV-1 inducer of short transcripts binding protein, HIV-1 inducer of short transcripts-binding factor 1, Leukemia/ly |
| Swissprot | |
| Calculated MW | 61 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus. |
| Concentration | 1.8 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | LRF, formerly identified as Pokemon, is a poxvirus zinc finger (POZ) domain-containing transcription factor that influences cell differentiation.LRF (for leukemia/lymphoma related factor) is also known as zinc finger and BTB domain containing 7A, ZBTB7, TIP21, FBI1 and FBI-1.POZ-domain transcription factors contain a POZ or BTB type protein-protein interaction domain at their N-terminus and Kruppel-type zinc fingers at their C-terminus.LRF is inducible during both murine and human preadipocyte differentiation and may contrib-ute to adipogenesis through influencing the switch from cellular proliferation to terminal differentiation. |
Other Clones
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Unconjugated
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