Xanthine Oxidase (XOD) Activity Fluorometric Assay Kit (E-BC-F019)
For research use only.
| Detection Principle | Hypoxanthine are oxidized by xanthine oxidase (XOD) to produce xanthine and super oxygen anion, which will quickly converte to hydrogen peroxide in the system, and then, in the role of peroxidase, hydrogen peroxide can oxidize the non-fluorescent probe to fluorescent substance. By measuring the fluorescence value, the corresponding the activity of xanthine oxidase can be calculated. |
| Synonyms | XOD |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Oxidative Stress |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.01 U/L |
| Detection Range | 0.01-1.2 U/L |
| Precision | inter-assay CV: 0.09 | intra-assay CV: 4.1% |
| Sample Volume | 100 μL |
| Assay Time | 25 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Mouse serum | 1 |
| Dog serum | 3-5 |
| Rat plasma | 1 |
| Horse serum | 1 |
| Human plasma | 1 |
| 10% Rat kidney tissue homogenate | 5-10 |
| 10% Mouse heart tissue homogenate | 5-10 |
| 10% Rat lung tissue homogenate | 5-10 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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