For research use only.
| Verified Samples | Verified Samples in WB: Mouse kidney Verified Samples in IHC: Human cervical cancer |
| Dilution | WB 1:500-1:2000, IHC 1:30-1:150 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human WDFY2 |
| Abbre | WDFY2 |
| Synonyms | PROF, Propeller-FYVE protein, RP11 147H23.1, WD repeat and FYVE domain containing 2, WD repeat and FYVE domain-containing protein 2, WD40 and FYVE domain containing 2, WD40- and FYVE domain-containing protein 2, WDF2, WDFY2, Wdfy2, ZFYVE22, Zinc finger FYVE domain-c |
| Swissprot | |
| Calculated MW | 45 kDa |
| Observed MW | Refer to figures The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 0.6 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Signal transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | WD repeat and FYVE domain-containing protein 2 (WDFY2), also known as WDF2 and ZFYVE22, is a 400 amino acid protein that localizes to a set of small endosomes that are found within 100 nm from the plasma membrane. Highly conserved between species, WDFY2 consists of one FYVE-type zinc finger and seven WD repeats. The FYVE domain is a cysteine-rich domain of about 70 amino acids. Its primary role is to target signal-transducing proteins to cell membranes through binding to the membrane lipid phosphatidylinositol-3-phosphate with high specificity. WD-repeats are generally found in clusters of seven. They have no intrinsic catalytic activity, but they serve as a platform for protein-protein interactions. WDFY2 is suspected to play a critical role in the endocytic pathway. |
Other Clones
1 Results
Other Formats
1 Results
Unconjugated
