VTI1B Polyclonal Antibody (E-AB-52425)
For research use only.
| Verified Samples |
Verified Samples in WB: Human kidney, RAW264.7, Mouse kidney, Human lung, Human fetal liver, Hela, 231 Verified Samples in IHC: Human liver cancer, Human esophagus cancer |
| Dilution | WB 1:500-1:2000, IHC 1:25-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Full length fusion protein |
| Abbre | VTI1B |
| Synonyms | HGNC:17793, V SNARE, Vesicle associated soluble NSF attachment protein receptor, Vesicle transport through interaction with , Vesicle transport through interaction with t SNAREs 1B, Vesicle transport through interaction with t SNAREs homolog 1B (yeast), v-SNARE |
| Swissprot | |
| Calculated MW | 27 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Endomembrane system. Cytoplasmic granule. |
| Concentration | 0.4 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | VTI1B (Vesicle Transport Through Interaction With T-SNAREs 1B) is a Protein Coding gene.Diseases associated with VTI1B include Hypomyelinating Leukodystrophy.Among its related pathways are wtCFTR and deltaF508 traffic / Late endosome and Lysosome (norm and CF) and Response to elevated platelet cytosolic Ca2+.Gene Ontology (GO) annotations related to this gene include SNARE binding and chloride channel inhibitor activity.An important paralog of this gene is VTI1A. |
Other Clones
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Unconjugated
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