Vitamin E (VE) Colorimetric Assay Kit (E-BC-K033-S)
For research use only.
| Detection Principle | Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. VE content can be calculated by measuring the OD value at 532 nm. |
| Synonyms | VE |
| Sample Type | Serum,Plasma,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (533 nm) |
| Research Area | Oxidative Stress , Plant Physiology Series |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4), Absolute ethanol, N-heptane |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.09 μg/mL |
| Detection Range | 0.09-40 μg/mL |
| Precision | inter-assay CV: 4.9% | intra-assay CV: 4.4% |
| Sample Volume | 0.3 mL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| 10% Mouse liver tissue homogenization | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| 10% Carrot tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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