For research use only.
| Verified Samples | Verified Samples in WB: 293T, Hela, Jurkat, A431, Human heart, Mouse kidney, HepG2 Verified Samples in IHC: Human tonsil |
| Dilution | WB 1:500-1:2000, IHC 1:25-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Full length fusion protein |
| Abbre | VDAC1 |
| Synonyms | Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin, Porin 31HL, Porin 31HM, VDAC, VDAC 1, VDAC-1, VDAC1, Vdac1, Voltage Dependent Anion Channel 1, Voltage dependent anion selective channel protein 1, Voltage-dependent anion-selective chann, hVDAC1 |
| Swissprot | |
| Calculated MW | 31 kDa |
| Observed MW | Refer to figures The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Mitochondrion outer membrane. Cell membrane. |
| Concentration | 0.6 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Metabolism, Signal Transduction, Tags and Cell Markers |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | VDAC1,also named as VDAC,Porin 31HM,Porin 31HL and Plasmalemmal porin,belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV,to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins,porins are large enough to allow passive diffusion. |
Other Clones
1 Results
Other Formats
1 Results
Unconjugated
