Uricase Acitivity Colorimetric Assay Kit (E-BC-K896-M)

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All	Size	Price	Qty
	96T	$ 420.00
	48T	$ 280.00

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Detection Principle	Uricase is an enzyme in the purine decomposition pathway, which can specifically catalyze the oxidation of uric acid into allantoin with higher solubility, thereby reducing the blood uric acid level. The detection principle of this kit is: uricase can oxidize the substrate, and the substrate has the maximum absorption peak at 293 nm. The uricase activity was calculated by measuring the OD value reduction at 293 nm.
Sample Type	Animal tissue,Plant tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (optimum wavelength: 293 nm)
Research Area	Liver And Renal Function
Other Reagents Required	PBS (0.01 M, pH 7.4)
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.06 U/L
Detection Range	0.06-4.00 U/L
Precision	inter-assay CV: 6.7-8.8% \| intra-assay CV: 2.6-3.4%
Sample Volume	40 μL (tissue homogenate)
Assay Time	50 min

Cat.No.	Product Name	Sizes
E-BC-K016-M	Uric Acid (UA) Colorimetric Assay Kit	96T , 48T
E-BC-K183-M	Urea (BUN) Colorimetric Assay Kit (Urease Method)	96T
E-BC-K188-M	Creatinine (Cr) Colorimetric Assay Kit (Sarcosine Oxidase Method)	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Mouse liver tissue homogenate	1-3
10% Porcine liver tissue homogenate	1-3
10% Spinach tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.