Uric Acid (UA) Colorimetric Assay Kit (E-BC-K016-S)
For research use only.
| Detection Principle | Uric acid can be used as an antioxidant to remove peroxide, hydroxyl and oxygen free radicals, chelate and transfer metal ions, protect vascular endothelial cells from damage. Uric Acid in protein-free filtrate reduce phosphotungstic acid to form tungsten blue, allantoin and carbon dioxide, the depth of blue color is proportional to the concentration of uric acid. |
| Synonyms | UA |
| Sample Type | Serum,Plasma,Urine |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (690 nm) |
| Research Area | Liver And Renal Function |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.58 mg/L |
| Detection Range | 0.58-100 mg/L |
| Precision | inter-assay CV: 2.6% | intra-assay CV: 1.8% |
| Sample Volume | 200 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human urine | 8-10 |
| Human serum | 1 |
| Dog serum | 1 |
| Rat serum | 1 |
| Mouse serum | 1-2 |
| Porcine serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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