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For research use only.
Product Summary
The new series of Elabscience® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.
| Sensitivity | 0.21pg/mL |
| Detection Range | 1.56-100 pg/mL |
| Sample Volume | 100 μL |
| Manual Operation Time | 1 h |
| Total Assay Time | 24h |
| Reacitivity | Rat |
| Specificity | This kit recognizes REN in samples.No significant cross-reactivity or interference between REN and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T*5 / 96T*15 |
| Storage | -20℃ |
| Expiration Date | 12 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to REN . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for REN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain REN , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of REN . You can calculate the concentration of REN in the samples by comparing the OD of the samples to the standard curve.
Background
Renin is a secreted endopeptidase that plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen,which is the only known physiological substrate of Renin. The 406 aa precursor includes a signal peptide (aa 1 - 23),a propeptide (aa 24 - 66),and a mature chain (aa 67 - 406). It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. Renal Renin (Renin-1) is synthesized as a 381 aa proform (aa 22 - 402). In the kidney,prorenin is proteolytically cleaved after Thr71 to generate a mature enzyme.
| Gene Alias | Ren |
| Gene ID | 24715 |
| Uniport ID | P08424 |
| Protein Alias | Ren |
| Research Area | Signal Transduction, Cardiovascular |
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