Uncoated Rat LBP ( Lipopolysaccharide Binding Protein ) ELISA Kit (E-UNEL-R0087)

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	96T*5	$ 399.00
	96T*15	$ 958.00

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Sensitivity	0.45ng/mL
Detection Range	1.56-100 ng/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	24h
Reacitivity	Rat
Specificity	This kit recognizes LBP in samples.No significant cross-reactivity or interference between LBP and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LBP . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LBP , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of LBP . You can calculate the concentration of LBP in the samples by comparing the OD of the samples to the standard curve.

Background

LPS-binding protein (LBP) is a protein that binds to the lipid A moiety of bacterial lipopolysaccharides (LPS),which is a glycolipid present in the outer membrane of all Gram-negative bacteria. It is involved in the immunological response to infections of Gram-negative bacteria,together with bactericidal permeability-increasing protein (BPI). LBP also interacts with the CD14 receptor and enhances its association with LPS. LBP is associated with diseases including bacterial sepsis,and is related to cytokine release in response to bacteria in immune system.

Gene Alias	Lbp
Gene ID	29469
Uniport ID	Q63313
Protein Alias	Lbp
Research Area	Cell Biology , Immunology , Neuroscience

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