Uncoated Rat CXCL16 ( Chemokine C-X-C-Motif Ligand 16 ) ELISA Kit (E-UNEL-R0084)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CXCL16 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CXCL16 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CXCL16 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CXCL16 . You can calculate the concentration of CXCL16 in the samples by comparing the OD of the samples to the standard curve.

CXCL16,a 27 kD chemokine,exists in membrane-bound and soluble forms.  Unlike classic chemokines,CXCL16 possesses a mucin-like stalk,a transmembrane domain,and a cytoplasmic tail with a tyrosine phosphorylation domain,allowing it to be expressed on the cell surface.  Structurally,it is similar to fractalkine.  It is expressed by dendritic cells,monocytes,and T cells.  T cells can also migrate in response to CXCL16.