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Uncoated Rat ACE ( Angiotensin Ⅰ Converting Enzyme ) ELISA Kit (E-UNEL-R0086)

All Size Price Qty
96T*5 $ 399.00
96T*15 $ 958.00
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For research use only.

Product Summary

The new series of Elabscience® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Sensitivity 0.05ng/mL
Detection Range 0.31-20 ng/mL
Sample Volume 100 μL
Manual Operation Time 1 h
Total Assay Time 24h
Reacitivity Rat
Specificity This kit recognizes ACE in samples.No significant cross-reactivity or interference between ACE and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T*5 / 96T*15
Storage -20℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ACE . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ACE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ACE , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ACE . You can calculate the concentration of ACE in the samples by comparing the OD of the samples to the standard curve.
CD143 (ACE,kininase II,peptidyl dipeptidase 1,peptidase P,carboxycathepsin) is a 171 kD,type I,single chain transmembranal metallopeptidase,whose cofactor is zinc. Its main targets are angiotensin I and bradykinin,acting as a blood pressure regulator. CD143 is expressed in endothelial cells, varying amounts of CD143 have been reported in different epithelial cells. The activation of macrophages and histiocytes induces the expression of this molecule.
Gene Alias Ace
Gene ID 24310
Uniport ID P47820
Protein Alias Ace
Research Area Immunology
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