Uncoated Mouse CD48 ( Cluster Of Differentiation 48 ) ELISA Kit (E-UNEL-M0177)

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	96T*5	$ 399.00
	96T*15	$ 958.00

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Sensitivity	6.32pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	24h
Reacitivity	Mouse
Specificity	This kit recognizes CD48 in samples.No significant cross-reactivity or interference between CD48 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CD48 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CD48 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CD48 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CD48 . You can calculate the concentration of CD48 in the samples by comparing the OD of the samples to the standard curve.

Background

Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with cell surface receptors including 2B4/CD244 or CD2 to regulate immune cell function and activation. Participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex. In turn,promotes LCK phosphorylation and subsequent activation. Participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex. In turn,promotes LCK phosphorylation and subsequent activation.

Gene Alias	Cd48
Gene ID	12506
Uniport ID	P18181
Protein Alias	Cd48
Research Area	Immunology

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