Uncoated Human TNF-β ( Tumor Necrosis Factor Beta ) ELISA Kit (E-UNEL-H0389)

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All	Size	Price	Qty
	96T*5	$ 399.00
	96T*15	$ 958.00

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Sensitivity	3.81pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	24h
Reacitivity	Human
Specificity	This kit recognizes TNF-β in samples.No significant cross-reactivity or interference between TNF-β and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TNF-β . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TNF-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TNF-β , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TNF-β . You can calculate the concentration of TNF-β in the samples by comparing the OD of the samples to the standard curve.

Background

LT-α,also known as TNF-β,is a potent mediator of inflammatory and immune responses. It belongs to the TNF superfamily of ligands and signals through TNFR1 and TNFR2. LT-α is produced by activated T and B lymphocytes,and has similar activities to TNF-α. Like TNF-α,LT-α (TNF-β) is involved in the regulation of various biological processes including cell proliferation,differentiation,apoptosis,lipid metabolism,coagulation,and neurotransmission. LT-α is secreted as a soluble polypeptide,but can form heterotrimers with LT-β,which effectively anchors the LT-α to the cell surface. LT-α is cytotoxic to a wide range of tumor cells.

Gene Alias	LTA
Gene ID	4049
Uniport ID	P01374
Protein Alias	LTA
Research Area	Cell Biology , Immunology , Neuroscience

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