Uncoated Human SCFR ( Stem Cell Growth Factor Receptor ) ELISA Kit (E-UNEL-H0379)

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	96T*5	$ 399.00
	96T*15	$ 958.00

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Sensitivity	10.83pg/mL
Detection Range	62.50-4000 pg/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	24h
Reacitivity	Human
Specificity	This kit recognizes SCFR in samples.No significant cross-reactivity or interference between SCFR and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SCFR . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SCFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SCFR , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of SCFR . You can calculate the concentration of SCFR in the samples by comparing the OD of the samples to the standard curve.

Background

CD117 is a 145 kD immunoglobulin superfamily member,also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor,stem cell factor,and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells,progenitors committed to myeloid and/or erythroid lineages,and T and B cell precursors),mast cells,and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Gene Alias	KIT
Gene ID	3815
Uniport ID	P10721
Protein Alias	KIT
Research Area	Immunology , Stem Cells

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