Uncoated Human RANκL ( Receptor Activator of Nuclear Factor Kappa B Ligand ) ELISA Kit (E-UNEL-H0385)

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	96T*5	$ 399.00
	96T*15	$ 958.00

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Sensitivity	82.71pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	24h
Reacitivity	Human
Specificity	This kit recognizes RANκL in samples.No significant cross-reactivity or interference between RANκL and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to RANκL . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RANκL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain RANκL , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of RANκL . You can calculate the concentration of RANκL in the samples by comparing the OD of the samples to the standard curve.

Background

RANKL gene encodes a type II membrane protein of 316 amino acids with a predicted molecular mass of 35 kD. RANKL is cleaved to produce a soluble form with biological activity. The shedding of membrane-bound RANKL appears to be mediated by expression of MMP14 and ADAM10. Suppression of MMP14 in primary osteoblasts increases membrane-bound RANKL and promotes osteoclastogenesis in cocultures with macrophages. Therefore,RANKL shedding seems to be an important process that down-regulates local osteoclastogenesis. Alternatively,an increased production of RANKL by osteoblastic cells leads to osteoclast differentiation,activation,and survival,which results in increased bone resorption. Binding of RANKL to its receptor RANK activates TNF receptor-associated factor 6 (TRAF6),which is linked to downstream pathways including NF-κB,c-jun N-terminal kinase (JNK) or Src.

Gene Alias	TNFSF11
Gene ID	21943
Uniport ID	O14788
Protein Alias	TNFSF11
Research Area	Immunology , Stem Cells

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