Uncoated Human MIP-3α ( Macrophage Inflammatory Protein 3 Alpha ) ELISA Kit (E-UNEL-H0386)

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	96T*5	$ 399.00
	96T*15	$ 958.00

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Sensitivity	4.39pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	24h
Reacitivity	Human
Specificity	This kit recognizes MIP-3α in samples.No significant cross-reactivity or interference between MIP-3α and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MIP-3α . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MIP-3α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MIP-3α , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of MIP-3α . You can calculate the concentration of MIP-3α in the samples by comparing the OD of the samples to the standard curve.

Background

CCL20 (MIP-3a) belongs to the CC chemokine family. Human CCL20 is a protein of 96 amino acids,which shares the highest homology of 28% amino acid identity with MIP-1ß and 64% amino acid sequence identity with the mouse CCL20. CCL20 is produced by activated monocytes,T cells,neutrophils,endothelial cells,epithelial cells and fibroblasts. CCL20 is the ligand for chemokine receptor CCR6,which is expressed on memory T cells,B cells,and immature dendritic cells (DCs). Therefore,it is involved in the regulation of DC trafficking and recruitment and activation of T cells. CCL20 also has potent antimicrobial activity. Upregulation of CCL20 plays important roles in autoimmune pathogenesis of the central nerve system (MS),pathogenesis of encephalomyelitis,psoriasis,rheumatoid arthritis,and inflammatory bowel diseases. CCL20 is also involved in various cancers,such as gastrointestinal cancers,pancreatic cancer,hepatocellular carcinoma,and breast cancer.

Gene Alias	CCL20
Gene ID	6364
Uniport ID	P78556
Protein Alias	CCL20
Research Area	Cell Biology , Signal Transduction

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