Uncoated Human MCP-4 ( Monocyte Chemotactic Protein 4 ) ELISA Kit (E-UNEL-H0378)

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All	Size	Price	Qty
	96T*5	$ 399.00
	96T*15	$ 958.00

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Sensitivity	51.55pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	24h
Reacitivity	Human
Specificity	This kit recognizes MCP-4 in samples.No significant cross-reactivity or interference between MCP-4 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MCP-4 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MCP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MCP-4 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of MCP-4 . You can calculate the concentration of MCP-4 in the samples by comparing the OD of the samples to the standard curve.

Background

Human CCL13 (MCP-4) was initially identified in a library constructed from human fetal mRNA. CCL13 shares a high similarity with CCL2 (MCP-1,61%),CCL7 (MCP-3,58%),and CCL8 (MCP-2,55%). CCL13 is constitutively expressed at high levels in the small intestine,colon,and lung. Original data showed that CCL13 was less effective than MCP-1 and MCP-3 in monocyte and lymphocyte chemoattraction,but equivalent to eotaxin as a chemoattractant for eosinophils. Chemokines are very important in the inflammatory process,initially in the influx of PMN by CXC chemokines and subsequently chemoattracting monocytes where the MCPs participate,including CCL13. The bioactivity of pro-inflammatory chemokines is regulated by MMPs in order to regulate the influx of different cell subpopulations.

Gene Alias	CCL13
Gene ID	6357
Uniport ID	Q99616
Protein Alias	CCL13
Research Area	Cell Biology , Signal Transduction

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