Uncoated Human ES ( Endostatin ) ELISA Kit (E-UNEL-H0382)

Citations ({{ citationsPage.numTotal }}) Manual MSDS

All	Size	Price	Qty
	96T*5	$ 399.00
	96T*15	$ 958.00

Bulk request >>

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Sensitivity	0.12ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	24h
Reacitivity	Human
Specificity	This kit recognizes ES in samples.No significant cross-reactivity or interference between ES and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ES . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ES , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ES . You can calculate the concentration of ES in the samples by comparing the OD of the samples to the standard curve.

Background

Endostatin is a C-terminal proteolytic fragment of collagen XVIII α1 (Col 18) that is localized in the vascular basement membrane of almost all epithelia and endothelia. Endostatin is produced from catalytic cleavage from MMPs,cathepsin L,and S. It is detected in circulation at a physiologic level of 20 to 50 ng/ml in serum. Endostatin interacts with multiple molecules including heparin,heparan sulfate,chondroitin sulfate,dermatan sulfate,thrombospondin-1,SPARC,collagens (I,IV,and VI),the amyloid peptide Aβ (1-42),transglutaminase-2,and among others. Endostatins derived from collagens XV and XVIII differ in structure and binding properties,tissue distribution,and anti-angiogenic activity. Endostatin is an endogenous angiostatic factor that induces apoptosis in endothelial cells and inhibits endothelial cell migration in vitro.

Gene Alias	COL18A1
Gene ID	80781
Uniport ID	P39060
Protein Alias	COL18A1
Research Area	Cell Biology

Other Clones

{{antibodyDetailsPage.numTotal}} Results

All

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS

Cat.No.：{{item.cat}}

{{index}} {{goods_show_value}}

Other Formats

{{formatDetailsPage.numTotal}} Results

All

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS

Cat.No.：{{item.cat}}

{{index}} {{goods_show_value}}

IF：{{item.impact}}

Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}
Reactivity：{{item.species}}

Sample Type：{{item.organization}}

Previous
{{ page }}
Next

Species

{{relatedElisaPage.numTotal}} Results

All

Human

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS

Cat.No.：{{item.cat}}

{{index}} {{goods_show_value}}

Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}

Previous
{{ page }}
Next