Uncoated Human CD38 ( Cyclic ADP Ribose Hydrolase ) ELISA Kit (E-UNEL-H0390)

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	96T*5	$ 399.00
	96T*15	$ 958.00

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Sensitivity	4.21pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	24h
Reacitivity	Human
Specificity	This kit recognizes CD38 in samples.No significant cross-reactivity or interference between CD38 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CD38 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CD38 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CD38 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CD38 . You can calculate the concentration of CD38 in the samples by comparing the OD of the samples to the standard curve.

Background

CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain,muscle,and kidney). In humans,it is expressed at high levels on plasma cells and activated T and B cells,natural killer (NK) lymphocytes,myeloblasts,and erythroblasts. By functioning as both a cyclase and a hydrolase,CD38 mediates lymphocyte activation,adhesion,and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.

Gene Alias	CD38
Gene ID	12494
Uniport ID	P28907
Protein Alias	CD38
Research Area	Cell Biology , Immunology

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