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Uncoated Human ADAM12 ( A Disintegrin And Metalloprotease 12 ) ELISA Kit (E-UNEL-H0388)

All Size Price Qty
96T*5 $ 399.00
96T*15 $ 958.00
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For research use only.

Product Summary

The new series of Elabscience® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Sensitivity 0.01ng/mL
Detection Range 0.16-10 ng/mL
Sample Volume 100 μL
Manual Operation Time 1 h
Total Assay Time 24h
Reacitivity Human
Specificity This kit recognizes ADAM12 in samples.No significant cross-reactivity or interference between ADAM12 and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T*5 / 96T*15
Storage -20℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ADAM12 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ADAM12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ADAM12 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ADAM12 . You can calculate the concentration of ADAM12 in the samples by comparing the OD of the samples to the standard curve.
Involved in skeletal muscle regeneration,specifically at the onset of cell fusion.  Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. Isoform 1 is expressed in placenta and skeletal,cardiac,and smooth muscle.  Isoform 2 seems to be expressed only in placenta or in embryo and fetus.  Both forms were expressed in some tumor cells lines.
Gene Alias ADAM12
Gene ID 8038
Uniport ID O43184
Protein Alias ADAM12
Research Area Signal Transduction , Cancer
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