Tyrosine Ammonia-lyase (TAL) Activity Assay Kit (E-BC-K851-M)
For research use only.
| Detection Principle | TAL can decompose tyrosine to produce 4-coumaric acid, which has a strong absorption peak at 333 nm. Therefore, the activity of TAL can be calculated by measuring the OD value at 333 nm. |
| Synonyms | TAL |
| Sample Type | Fruit juices,Plant,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (323-343 nm, optimum wavelength: 333 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.12 U/mL |
| Sample Volume | 80 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 20% Epipremnum aureum tissue homogenate | 1 |
| 20% Celery tissue homogenate | 1 |
| 20% Cilantro tissue homogenate | 1 |
| 20% Rat liver tissue homogenate | 1 |
| 20% Rat kidney tissue homogenate | 1 |
| 20% Corn tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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