Trehalose Colorimetric Assay Kit (Enzyme Method) (E-BC-K754-M)
For research use only.
| Detection Principle |
Trehalose, a non-reducing disaccharide composed of two glucose molecules, is widely found in lower plants, invertebrates and microorganisms. Trehalose is the main energy storage substance for many insects and fungi. In addition, trehalose has a non-specific protective effect on organisms and biological macromolecules. This special property makes it widely used in many fields such as cosmetics, food industry, medicine and agriculture. This kit specifically hydrolyzes trehalose into two molecules of glucose by trehalose enzyme, and then detects the glucose content by the GOD-POD method. Control well is set up to eliminate the influence of the glucose background value, thereby determining the trehalose content in the sample. It has the characteristics of strong specificity and high sensitivity. |
| Sample Type | Fungus,Algae,Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (500-520 nm, optimum wavelength: 510 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Plant Physiology Series , Energy Metabolism |
| Storage | 该产品可在 2-8°C避光条件下保存6个月。 |
| Valid Period | 12 months |
| Sensitivity | 0.007 mg/mL |
| Detection Range | 0.007-1.00 mg/mL |
| Precision | inter-assay CV: 1.9-6.6% | intra-assay CV: 1.3-2.6% |
| Sample Volume | 20 μL(tissue homogenate) |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Pleurotus djamor pileus tissue homogenate | 8-16 |
| 10% Pleurotus ostreatus pileus tissue homogenate | 5-10 |
| 10% Lentinus edodes pileus tissue homogenate | 2-4 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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