Trehalose Colorimetric Assay Kit (E-BC-K754-M)
For research use only.
| Detection Principle |
Trehalose is found in a large number of organisms, including bacteria, algae, yeast, plants, insects, and other invertebrates. Because trehalose has unique biological characteristics different from other carbohydrates, it can protect the protein, lipid, carbohydrate, nucleic acid and other components of biological cells from damage under the harsh environment such as drought, high temperature, dehydration, freezing, high osmotic pressure and toxic substances. The determination method was a highly specific enzyme method, trehalase was used to hydrolyze trehalose into 2 molecules of glucose, and then the glucose content was detected by GOD-POD method. Other disaccharides such as maltose and lactose did not interfere with the determination. |
| Sample Type | Serum,Plasma,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (505-515 nm, optimum wavelength: 510 nm) |
| Research Area | Plant Physiology Series , Metabolic Diseases |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 0.05 mg/mL |
| Detection Range | 0.05-1.9 mg/mL |
| Precision | inter-assay CV: 4.5-5.6% | intra-assay CV: 2.1-4.0% |
| Sample Volume | 20 μL |
| Assay Time | 1 h |
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® Violet 450
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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