Transglutaminases (TGs) Activity Colorimetric Assay Kit (E-BC-K840-M)
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For research use only.
| Detection Principle | Transglutaminases (TG) catalyze the transfer of acyl groups between glutamine residues and lysine residues or primary amines. TG-mediated protein crosslinking is essential for a variety of biological processes and diseases. TG is also widely used as a food processing aid in meat, fish, dairy and grain products. The detection principle of this kit is: TG catalyzes the acyl transfer between glutamine residues and primary amines, the product forms a chromogenic substance with the chromogenic agent, and determines the activity of TG in the sample by measuring the absorbance change at 525 nm. |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (520-530 nm, optimum wavelength: 525 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 3.0 U/L |
| Detection Range | 3.0-25.0 U/L |
| Precision | inter-assay CV: 5.0-6.1% | intra-assay CV: 2.0-2.9% |
| Sample Volume | 120 μL |
| Assay Time | 1 h 40 min |
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Biotin
