Total Sulfhydryl Group/Total Thiol (-SH) Colorimetric Assay Kit (E-BC-K265-M)
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| Detection Principle | Sulfhydryl compounds react with 5,5' -dithiobis (2-nitrobenzoic acid) under neutral or alkaline conditions to produce a yellow product which have a maximum absorption peak at 412 nm. Measure the OD value and calculate the total mercapto content indirectly. |
| Synonyms | T-SH |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (410-420 nm, optimum wavelength: 412 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Absolute ethyl alcohol,Normal saline (0.9% NaCl) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 9.91 μmol/L |
| Detection Range | 9.91-1000 μmol/L |
| Precision | inter-assay CV: 2.9% | intra-assay CV: 2.5% |
| Sample Volume | 80 μL |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 4-6 |
| Human plasma | 4-6 |
| Human urine | 1 |
| Rabbit serum | 3-5 |
| Porcine serum | 4-6 |
| 10% Rat liver tissue homogenate | 4-6 |
| 10% Rat kidney tissue homogenate | 4-6 |
| 10% Rat spleen tissue homogenate | 4-6 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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