Total Phenols Colorimetric Assay Kit (Plant samples) (E-BC-K354-M)

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	500Assays	$ 720.00
	96T	$ 240.00

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Detection Principle	Under alkaline conditions, tungsten-molybdenum acid can be reduced by phenols and produce blue compounds, which has a characteristic absorption peak at 760 nm. The content of total phenols in sample can be calculated indirectly by measuring the absorbance at 760 nm.
Sample Type	Plant tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (750-770 nm, optimum wavelength: 760 nm)
Research Area	Plant Physiology Series
Other Reagents Required	60% Absolute ethanol
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	1.05 μg/mL
Detection Range	1.05-148 μg/mL
Precision	inter-assay CV: 4.4% \| intra-assay CV: 4.1%
Sample Volume	20 μL(tissue homogenate)
Assay Time	35 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Epipremnum aureum tissue homogenate	20-30
Daucus carota tissue homogenate	5-15
Spinacia oleracea tissue homogenate	15-25
Leek tissue homogenate	10-20

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.