Total Iron Binding Capacity (TIBC) Colorimetric Assay Kit (E-BC-K071-M)
For research use only.
| Detection Principle | The excess iron is added to the serum to bind all the ferritin in the serum, and the excess iron is adsorbed by adding the iron adsorbent. The iron bind with the ferritin is separated from the protein by the action of acid solution and reductant. Fe3+ in serum is reduced to Fe2+, Fe2+ binds with bipyridine to form pink complex. In a certain range, the amount of TIBC is positively correlated with the depth of color. The iron content measured is, minus serum iron value, which is called unsaturated iron binding force. Total iron binding capacity minus serum iron value is unsaturated iron binding capacity (UIBC). |
| Synonyms | TIBC |
| Sample Type | Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (510-530 nm, optimum wavelength: 520 nm) |
| Research Area | Inorganic Ions |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.14 mg/L |
| Detection Range | 0.31-50 mg/L |
| Precision | inter-assay CV: 2.3% | intra-assay CV: 1.5% |
| Sample Volume | 50 μL |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat serum | 1 |
| Porcine serum | 1 |
| Rabbit serum | 1 |
| Chicken serum | 1 |
| Machin serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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