Total Iron Binding Capacity (TIBC) And Serum Iron Assay Kit (Direct Method) (E-BC-K945-M)
For research use only.
| Detection Principle |
The amount of iron bound to transferrin in the blood is called serum iron. The maximum ability of transferrin to bind iron is called total iron binding capacity (TIBC), and iron saturation refers to the percentage of serum iron binding capacity to total iron binding capacity. The detection principle of this kit: add an excess of iron to the sample and detect the total iron content. The content of unbound iron was detected when transferrin was saturated with iron. The total iron binding capacity of the sample is obtained by subtracting the content of unbound iron from the total iron content. The sample is not added with iron, and the total content of iron bound to transferrin and free iron is detected. Meanwhile, the content of free iron that has not bound to transferrin is detected. The serum iron content is obtained by subtracting the latter from the former. |
| Sample Type | Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (560-580 nm, optimum wavelength: 570 nm) |
| Research Area | Metabolic Diseases , Liver And Renal Function , Inorganic Ions |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.684 μmol/L |
| Detection Range | 1.446-500 μmol/L |
| Precision | inter-assay CV: 2.1-2.7% | intra-assay CV: 0.3-0.6% |
| Sample Volume | 200 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat serum | 1 |
| Horse serum | 1 |
| Chicken serum | 1 |
| Rabbit serum | 1 |
| Mouse urine | 1 |
| Porcine serum | 1 |
| Fetal bovine serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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